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Journal / Cancer Research / ひと下垂体腺腫の組織のプロテオームの分析のための質量分析法と相まって二次元ゲル電気泳動…
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JoVE Journal Cancer Research
ひと下垂体腺腫の組織のプロテオームの分析のための質量分析法と相まって二次元ゲル電気泳動
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ひと下垂体腺腫の組織のプロテオームの分析のための質量分析法と相まって二次元ゲル電気泳動

Article DOI: 10.3791/56739-v • 12:34 min • April 2nd, 2018
April 2nd, 2018 •
1,2,3,4, 1,2,3, 1,2,3
1Key Laboratory of Cancer Proteomics of Chinese Ministry of Health, Xiangya Hospital, Central South University, 2Hunan Engineering Laboratory for Structural Biology and Drug Design, Xiangya Hospital, Central South University, 3State Local Joint Engineering Laboratory for Anticancer Drugs, Xiangya Hospital, Central South University, 4The State Key Laboratory of Medical Genetics, Central South University

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二次元ゲル電気泳動 (2DE) 質量分析 (MS) を人間の下垂体腺腫組織プロテオーム良いと再現可能な 2DE パターンを提示を区別して結合を紹介します。高感度 MS を使って複雑ながんのプロテオームを解析するとき、多くの蛋白質は各 2DE スポットで観察されます。

Tags

がん研究、問題 134、二次元ゲル電気泳動法、等電点焦点、アクリルアミドゲル電気泳動、銀染色、クマシー染色、画像解析、ペプチドマスフィンガー プリント (PMF)、タンデム質量分析 (MS/MS)、下垂体腺腫、プロテオーム
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