Journal
/
Developmental Biology
/
Handling and Treatment of Male European Eels (Anguilla anguilla) for Hormonal Maturation and Sperm Cryopreservation
JoVE Journal
Developmental Biology
Author Produced
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Developmental Biology
Handling and Treatment of Male European Eels (Anguilla anguilla) for Hormonal Maturation and Sperm Cryopreservation

Handling and Treatment of Male European Eels (Anguilla anguilla) for Hormonal Maturation and Sperm Cryopreservation

DOI:
10.3791/56835-v

•

08:24 min

•

January 28, 2018

•

, , , , , , , ,
1Grupo de Acuicultura y Biodiversidad, Instituto de Ciencia y Tecnología Animal,Universitat Politècnica de València, 2Department of Aquaculture, Institute of Aquaculture and Environmental Safety,Szent Istvan University

Chapters

  • 00:05Title
  • 00:11Introduction
  • 00:49Fish Maintenance and Hormonal Maturation
  • 01:59Samplings
  • 03:05Sperm Quality Evaluation
  • 04:21Sperm Freezing Solutions and Materials
  • 04:42Sperm Freezing Method
  • 05:26Thawing Method
  • 06:18Results
  • 07:09Conclusion

Summary

Automatic Translation

Automatic Translation

Protocols for European eel maturation and sperm cryopreservation have been improved over the last years. This article describes the best protocol available using human chorionic gonadotropin (hCG) for inducing maturation and methanol as cryoprotectant.

Tags

European EelAnguilla AnguillaHormonal MaturationSperm CryopreservationHCG InjectionsTanaka’s ExtenderMethanol CryoprotectantSperm Motility

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/56835?article_type=v

Manipulation and In Vitro Maturation of Xenopus laevis Oocytes, Followed by Intracytoplasmic Sperm Injection, to Study Embryonic Development
check_url/56835?article_type=v

Isolation and Cryopreservation of Neonatal Rat Cardiomyocytes
check_url/56835?article_type=v

Protocols for Analyzing the Role of Paneth Cells in Regenerating the Murine Intestine using Conditional Cre-lox Mouse Models
check_url/56835?article_type=v

Modified MicroSecure Vitrification: A Safe, Simple and Highly Effective Cryopreservation Procedure for Human Blastocysts
check_url/56835?article_type=v

Functional Manipulation of Maternal Gene Products Using In Vitro Oocyte Maturation in Zebrafish
check_url/56835?article_type=v

Maturation of Human Stem Cell-derived Cardiomyocytes in Biowires Using Electrical Stimulation
check_url/56835?article_type=v

A Standardized Approach for Multispecies Purification of Mammalian Male Germ Cells by Mechanical Tissue Dissociation and Flow Cytometry
check_url/56835?article_type=v

Induction and Validation of Cellular Senescence in Primary Human Cells
check_url/56835?article_type=v

Fluorimetric Techniques for the Assessment of Sperm Membranes
check_url/56835?article_type=v

Measuring Sperm Guidance and Motility within the Caenorhabditis elegans Hermaphrodite Reproductive Tract

Read Article