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JoVE Journal
Developmental Biology

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Fluorimetric Techniques for the Assessment of Sperm Membranes
 

Fluorimetric Techniques for the Assessment of Sperm Membranes

Article doi: 10.3791/58622
November 28th, 2018

Summary November 28th, 2018

Here, we present methodologies to evaluate spermatozoan membrane integrity, a cellular feature associated with sperm fertilization competence. We describe three techniques for the fluorimetric assessment of sperm membranes: simultaneous staining with specific fluorescent probes, fluorescence microscopy, and advanced sperm-dedicated flow cytometry. Examples of combining the methodologies are also presented.

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