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Developmental Biology

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Fluorimetric Techniques for the Assessment of Sperm Membranes

Fluorimetric Techniques for the Assessment of Sperm Membranes

Article DOI: 10.3791/58622-v 08:58 min November 28th, 2018
November 28th, 2018



Here, we present methodologies to evaluate spermatozoan membrane integrity, a cellular feature associated with sperm fertilization competence. We describe three techniques for the fluorimetric assessment of sperm membranes: simultaneous staining with specific fluorescent probes, fluorescence microscopy, and advanced sperm-dedicated flow cytometry. Examples of combining the methodologies are also presented.


Fluorimetric Techniques Sperm Membranes Fluorescent Probes Fluorescence Microscopy Flow Cytometry Analysis Fertilization Potential Sperm Sample Quality Sperm Plasma Membrane Acrosome Membrane Integrity Mitochondrial Membrane Potential Bull Semen NKM Buffer Centrifugation Supernatant Incubator Motile Spermatozoa Transfer
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