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神经入侵的体内小鼠坐骨神经模型
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Cancer Research
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JoVE Journal Cancer Research
An In Vivo Murine Sciatic Nerve Model of Perineural Invasion
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神经入侵的体内小鼠坐骨神经模型

DOI:
10.3791/56857-v

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09:55 min

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April 23, 2018

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, , , , , ,
1Department of Surgery,Memorial Sloan Kettering Cancer Center, 2Molecular Cytology Core Facility,Memorial Sloan Kettering Cancer Center, 3Department of Radiation Oncology,Mount Sinai Hospital

Chapters

  • 00:05Title
  • 01:25Preparation of Cultured Cancer Cells
  • 02:35Preparation of Mouse and Surgery
  • 06:06Sciatic Nerve Extraction
  • 07:24Nerve Processing and Quantification
  • 08:26Results: H&E Longitudinal Sections of Sciatic Nerves Injected with Panc02H7 Cancer Cells in Wild Type and NCAM KO Mice
  • 09:10Conclusion

Summary

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Automatic Translation

我们描述了一个体内小鼠神经入侵的模型, 注射自体胰腺癌细胞进入坐骨神经。该模型允许定量的神经入侵程度, 并支持调查的细胞和分子机制的神经入侵。

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