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Moving Upwards: A Simple and Flexible In Vitro Three-dimensional Invasion Assay Protocol
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1Department of Biomedical Engineering,University of Minnesota – Twin Cities, 2Stem Cell Institute,University of Minnesota – Twin Cities, 3Masonic Cancer Center,University of Minnesota – Twin Cities, 4Lillehei Heart Institute,University of Minnesota – Twin Cities, 5Institute for Engineering in Medicine,University of Minnesota – Twin Cities, 6Department of Biology,Jackson State University
Chapters
- 00:05Title
- 01:11Culture Plate, Collagen Gel Preparation, and Cell Imaging
- 03:33Results: Representative Inverted Vertical Invasion Assay Analyses
- 04:33Conclusion
This protocol describes an inverted vertical invasion assay that could be used to quantify the migration and invasion capabilities of cells in a three-dimensional setting while preserving the cell microenvironment. This assay is suitable for rare and/or sensitive cells.
