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Journal / Chemistry / Mikrofluid Chips for In Situ Crystal røntgen…
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Chemistry

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JoVE Journal Chemistry
Mikrofluid Chips for In Situ Crystal røntgen diffraktion og In Situ dynamisk lysspredning for serielle krystallografi
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Mikrofluid Chips for In Situ Crystal røntgen diffraktion og In Situ dynamisk lysspredning for serielle krystallografi

Article DOI: 10.3791/57133-v • 11:48 min • April 24th, 2018
April 24th, 2018 •
*1,2, *3,4,5, 3, 6, 6, 3,4, 3,4,5, 1,2,4, 1,7
1Center for Free Electron Laser Science, DESY, 2Department of Physics, University of Hamburg, 3Institute for Biochemistry and Molecular Biology, Laboratory for Structural Biology of Infection and Inflammation, University of Hamburg, 4The Hamburg Center for Ultrafast Imaging, University of Hamburg, 5Integrated Biology Infrastructure Life-Science Facility at the European XFEL (XBI), 6European Molecular Biology Laboratory, EMBL c/o DESY, 7Department of Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry
* These authors contributed equally

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Denne protokol beskriver i detaljer hvordan at fabrikere og drive mikrofluid enheder til røntgen diffraktion dataindsamling ved stuetemperatur. Desuden, den beskriver hvordan man kan overvåge protein krystallisering af dynamisk lysspredning og hvordan til at behandle og analysere opnået diffraktion data.

Tags

Kemi spørgsmål 134 i situ røntgen diffraktion faste mål seriel millisekund krystallografi mikrofluidik protein krystallisering i situ dynamiske lys spredning
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