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Journal / Chemistry / Neutron krystallografi Dataindsamling og…
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Neutron krystallografi Dataindsamling og behandling til modellering hydrogenatomer i proteinstrukturer
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Neutron krystallografi Dataindsamling og behandling til modellering hydrogenatomer i proteinstrukturer

Article DOI: 10.3791/61903-v • 10:10 min • December 1st, 2020
December 1st, 2020 •
1,2, 1,2
1Department of Molecular and Structural Biochemistry, North Carolina State University, 2Neutron Scattering Division, Oak Ridge National Laboratory

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Neutronproteinkrystallografi er en strukturel teknik, der tillader lokalisering af hydrogenatomer og derved giver vigtige mekanistiske detaljer om proteinfunktionen. Vi præsenterer her arbejdsgangen for montering af en proteinkrystal, neutron diffraktion dataindsamling, struktur raffinement og analyse af neutron spredning længde tæthed kort.

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Kemi Udgave 166 Neutronproteinkrystallografi neutroner proteiner hydrogenatomer protonation enzymer metalloproteiner strukturel biologi polysaccharidmonoxygenaser reaktionsmekanisme røntgenkrystallografi
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