Profiling DNA Replication Timing Using Zebrafish as an <em>In Vivo</em> Model System

Joseph C. Siefert; Emily A. Clowdus; Duane Goins; Amnon Koren; Christopher L. Sansam

doi:10.3791/57146

Journal

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Genetics

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Profilering DNA Replication Timing med zebrafiskar som ett In Vivo -modellsystem

Profiling DNA Replication Timing Using Zebrafish as an <em>In Vivo</em> Model System

JoVE Journal
Genetics

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JoVE Journal Genetics

Profiling DNA Replication Timing Using Zebrafish as an In Vivo Model System

Profilering DNA Replication Timing med zebrafiskar som ett In Vivo -modellsystem

Please note that all translations are automatically generated. Click here for the English version.

7,767 Views

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10:17 min

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April 30, 2018

DOI:

10.3791/57146-v

DOI:

10.3791/57146-v

•

10:17 min

April 30, 2018

•

15 Views

Joseph C. Siefert^1,2, Emily A. Clowdus^1,2, Duane Goins¹, Amnon Koren³, Christopher L. Sansam^1,2

¹Cell Cycle and Cancer Biology Research Program,Oklahoma Medical Research Foundation, ²Department of Cell Biology,University of Oklahoma Health Sciences Center, ³Department of Molecular Biology and Genetics,Cornell University

Transcript

Summary

Zebrafiskar användes nyligen som ett in vivo -modellsystem för att studera DNA-replikering tidpunkten under utveckling. Här är detaljerad protokollen för zebrafiskar embryon profil replikering timing. Detta protokoll kan enkelt anpassas för att studera replikering timing i mutanter, enskilda celltyper, sjukdomsmodeller och andra arter.