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Journal / Genetics / 使用斑马鱼作为体内模型系统分析 DNA…
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JoVE Journal Genetics
使用斑马鱼作为体内模型系统分析 DNA 复制定时
A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins
Journal (Genetics)
A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins
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Journal (Genetics)
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使用斑马鱼作为体内模型系统分析 DNA 复制定时

Article DOI: 10.3791/57146-v • 10:17 min • April 30th, 2018
April 30th, 2018 •
1,2, 1,2, 1, 3, 1,2
1Cell Cycle and Cancer Biology Research Program, Oklahoma Medical Research Foundation, 2Department of Cell Biology, University of Oklahoma Health Sciences Center, 3Department of Molecular Biology and Genetics, Cornell University

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斑马鱼最近被用作一个体内模型系统, 用于研究开发过程中 DNA 复制的时机。这里详细介绍了使用斑马鱼胚胎来描述复制时间的协议。该协议可以很容易地适应于研究突变体、个体细胞类型、疾病模型和其他物种的复制时间。

Tags

遗传学 问题 134 DNA 复制 复制时间 斑马鱼 在体内 发展 转录 染色质 下一代测序 拷贝数变异 突变 癌症 外地资产管制
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