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JoVE Journal
Biology

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共聚焦显微镜通过图像相关谱揭示细胞表面受体聚集
 
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共聚焦显微镜通过图像相关谱揭示细胞表面受体聚集

Article DOI: 10.3791/57164-v 06:51 min August 2nd, 2018
August 2nd, 2018

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Summary

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Click here for the English version.

与细胞表面上的靶受体结合的抗体可以赋予构象和聚类改变。这些动态变化对表征靶细胞内药物的发展有影响。该协议利用共焦显微和图像相关光谱学通过 ImageJ/斐济, 以量化的程度, 受体聚类在细胞表面。

Tags

生物学 问题 138 共焦显微镜 图像相关光谱学 (ICS) 抗体: 受体相互作用 抗体-药物共轭 (ADC) imageJ 斐济 图像处理 聚类 聚集
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