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共聚焦显微镜通过图像相关谱揭示细胞表面受体聚集
JoVE Journal
Biology
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JoVE Journal
Biology
Confocal Microscopy Reveals Cell Surface Receptor Aggregation Through Image Correlation Spectroscopy
Please note that all translations are automatically generated.
Click here for the English version.
共聚焦显微镜通过图像相关谱揭示细胞表面受体聚集
DOI:
10.3791/57164-v
•
06:51 min
•
August 02, 2018
•
Adam C. Parslow
2
,
Andrew H.A. Clayton
,
Peter Lock
,
Andrew M. Scott
2,5,6,7
1
Tumour Targeting Laboratory
,
Olivia Newton-John Cancer Research Institute
,
2
School of Cancer Medicine
,
La Trobe University
,
3
Centre for Micro-Photonics, Faculty of Science, Engineering and Technology
,
Swinburne University of Technology
,
4
LIMS Bioimaging Facility, La Trobe Institute for Molecular Science
,
La Trobe University
,
5
Department of Medical Oncology, Olivia Newton-John Cancer and Wellnes Centre
,
Austin Health
,
6
Department of Medicine
,
University of Melbourne
,
7
Department of Molecular Imaging and Therapy
,
Austin Health
Chapters
00:04
Title
00:39
Calculation of Image Correlation Spectroscopy
03:43
Batch Processing of ICS Datasets and Calculation of the Microscope s Beam Area
04:59
Results: Image Correlation Spectroscopy Detects Clusters on the Cell Surface of Adherent Cancer Cells
05:55
Conclusion
Summary
Automatic Translation
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Automatic Translation
与细胞表面上的靶受体结合的抗体可以赋予构象和聚类改变。这些动态变化对表征靶细胞内药物的发展有影响。该协议利用共焦显微和图像相关光谱学通过 ImageJ/斐济, 以量化的程度, 受体聚类在细胞表面。
Tags
Confocal Microscopy
Cell Surface Receptor
Aggregation
Image Correlation Spectroscopy
EGF Receptor
Immunocytochemistry
Image Processing
FD Math
Image Correlation
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