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Journal / Biology / Konfokalmikroskopi avslöjar Cell Surface Receptor …
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Konfokalmikroskopi avslöjar Cell Surface Receptor Aggregation genom bilden korrelation spektroskopi
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Konfokalmikroskopi avslöjar Cell Surface Receptor Aggregation genom bilden korrelation spektroskopi

Article DOI: 10.3791/57164-v • 06:51 min • August 2nd, 2018
August 2nd, 2018 •
1,2, 3, 4, 1,2,5,6,7
1Tumour Targeting Laboratory, Olivia Newton-John Cancer Research Institute, 2School of Cancer Medicine, La Trobe University, 3Centre for Micro-Photonics, Faculty of Science, Engineering and Technology, Swinburne University of Technology, 4LIMS Bioimaging Facility, La Trobe Institute for Molecular Science, La Trobe University, 5Department of Medical Oncology, Olivia Newton-John Cancer and Wellnes Centre, Austin Health, 6Department of Medicine, University of Melbourne, 7Department of Molecular Imaging and Therapy, Austin Health

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Konformation och klustring förändringar kan ge antikroppar som binder till rikta receptorer på cellytan. Dessa dynamiska förändringar påverkar för kännetecknar läkemedelsutveckling i målceller. Detta protokoll använder konfokalmikroskopi och bild korrelation spektroskopi genom ImageJ/FIJI att kvantifiera omfattningen av receptorn klustring på cellytan.

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Biologi fråga 138 konfokalmikroskopi bild korrelation spektroskopi (ICS) antikropp: receptor interaktioner antikropp-läkemedelskonjugat (ADC) imageJ Fiji bildbehandling klustring aggregering
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