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三次元ペプチド足場で哺乳動物の細胞を培養
JoVE Journal
Bioengineering
This content is Free Access.
JoVE Journal
Bioengineering
Culturing Mammalian Cells in Three-dimensional Peptide Scaffolds
Please note that all translations are automatically generated.
Click here for the English version.
三次元ペプチド足場で哺乳動物の細胞を培養
DOI:
10.3791/57259-v
•
07:52 min
•
June 13, 2018
•
Nausika Betriu
,
Lourdes Recha-Sancho
,
Carlos E. Semino
2
1
Tissue Engineering Research Laboratory, Department of Bioengineering, IQS-School of Engineering
,
Ramon Llull University
,
2
Hebe Biolab
Chapters
00:05
Title
00:51
Self-Assembling Peptide Scaffold (SAPS) Preparation
01:39
Cell Suspension Preparation
02:32
3D Cell Encapsulation into SAPS
04:52
Cell Viability in 3D-SAPS
05:55
Results: Attaining a 3D-Culture System in Self Assembling Peptide Scaffolds
07:03
Conclusion
Summary
Automatic Translation
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ここでは、自己組織化ペプチドのひと関節軟骨細胞の脱分化型軟骨への分化を促進する足場の 3 D 培養システムを取得するためのプロトコルを提案する.
Tags
3D Cell Culture
Peptide Scaffold
RAD16-I
Cell-cell Interaction
Cell-matrix Interaction
Cell Proliferation
Cell Migration
Cell Differentiation
Regenerative Medicine
Tumor Models
Diabetes
Neurodegenerative Diseases
Cell Suspension
Cell Concentration
Sonication
Centrifugation
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