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Genetics
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血栓烷 A2 受体基因 C924T 多态性的研究方法
JoVE Journal
Genetics
This content is Free Access.
JoVE Journal
Genetics
A Method to Study the C924T Polymorphism of the Thromboxane A2 Receptor Gene
Please note that all translations are automatically generated.
Click here for the English version.
血栓烷 A2 受体基因 C924T 多态性的研究方法
DOI:
10.3791/57289-v
•
07:00 min
•
April 01, 2019
•
Vincenzo De Iuliis
,
Sebastiano Ursi
,
Alfonso Pennelli
,
Marika Caruso
,
Sabrina Capodifoglio
,
Antonio Marino
,
Vincenzo Flati
,
Gianfranco Vitullo
,
Elena Toniato
,
Iole Robuffo
,
Stefano Martinotti
2
1
SS Annunziata University Hospital, Unit of Clinical Molecular Biology and Predictive Medicine
,
University of Chieti
,
2
Department of Medical, Oral and Biotechnological Sciences
,
University of Chieti
,
3
Department of Biotechnological and Applied Clinical Sciences
,
University of L’Aquila
,
4
CNR – Institute of Molecular Genetics, Section of Chieti
Chapters
00:04
Title
00:56
Reagent Setup
02:33
Purification and PCR Amplification of DNA Samples
03:39
RFLP of PCR Products Followed by Gel Electrophoresis of the PCR-RFLP Samples
04:44
Results: Analysis of RFLP to Identify the C924T Polymorphism in TBXA2R Gene
05:33
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在本研究中, 我们描述了一种分析 C924T 基因型的方法。该协议包括三个阶段: DNA 提取、聚合酶链反应扩增 (PCR) 和琼脂糖凝胶限制性片段长度多态性 (RFLP) 分析。
Tags
C924T Polymorphism
Thromboxane A2 Receptor Gene
Atherothrombosis
Personalized Medicine
Pharmacological Response
Tris EDTA Buffer
Electrophoresis Buffer
Agarose Gel
DNA Purification
DNA Quantification
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