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1SS Annunziata University Hospital, Unit of Clinical Molecular Biology and Predictive Medicine,University of Chieti, 2Department of Medical, Oral and Biotechnological Sciences,University of Chieti, 3Department of Biotechnological and Applied Clinical Sciences,University of L’Aquila, 4CNR – Institute of Molecular Genetics, Section of Chieti
Chapters
- 00:04Title
- 00:56Reagent Setup
- 02:33Purification and PCR Amplification of DNA Samples
- 03:39RFLP of PCR Products Followed by Gel Electrophoresis of the PCR-RFLP Samples
- 04:44Results: Analysis of RFLP to Identify the C924T Polymorphism in TBXA2R Gene
- 05:33Conclusion
En el presente estudio, se describe una metodología para analizar el genotipo C924T. El protocolo consta de tres etapas: extracción de ADN, amplificación por reacción en cadena de polimerasa (PCR) y análisis del polimorfismo de la longitud del fragmento de restricción (RFLP) en gel de agarosa.
