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JoVE Journal Chemistry
Co udtryk for flere kimære fluorescerende Fusion proteiner på en effektiv måde i planter
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Co udtryk for flere kimære fluorescerende Fusion proteiner på en effektiv måde i planter

Article DOI: 10.3791/57354-v • 09:45 min • July 1st, 2018
July 1st, 2018 •
*1, *1, 1
1College of Life Sciences, South China Agricultural University
* These authors contributed equally

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Vi har udviklet en ny metode til at co udtrykke flere kimære fluorescerende fusion proteiner i planter til at overvinde vanskelighederne af konventionelle metoder. Det tager fordel ved at bruge en enkelt udtryk plasmid som indeholder flere funktionelt uafhængige protein udtrykker kassetter for at opnå protein Co udtryk.

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Kemi sag 137 Protein Co udtryk kimære fluorescerende fusion protein protein subcellulært lokalisering og dynamik protein udtryk kassetter et-trins DNA forsamling reaktion enkelt udtryk vektor forbigående udtryk stabil genetiske transformation
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