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An Approach to Study Shape-Dependent Transcriptomics at a Single Cell Level
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Genetics
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JoVE Journal Genetics
An Approach to Study Shape-Dependent Transcriptomics at a Single Cell Level

An Approach to Study Shape-Dependent Transcriptomics at a Single Cell Level

DOI:
10.3791/61577-v

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06:02 min

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November 02, 2020

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1Department of Medicine, Integrated Cardio Metabolic Centre (ICMC), Heart and Vascular Theme,Karolinska Institutet, 2Bioscience Cardiovascular, Research and Early Development, Cardiovascular, Renal and Metabolism (CVRM), BioPharmaceuticals R&D,AstraZeneca

Chapters

  • 00:04Introduction
  • 00:30Cardiomyocyte (CM) Patterning
  • 01:41Adherent CM Picking
  • 04:57Results: Representative Shape-Dependent Transcriptomic Analyses
  • 05:38Conclusion

Summary

Automatic Translation

Automatic Translation

This paper presents methods for growing cardiac myocytes with different shapes, which represent different pathologies, and sorting these adherent cardiac myocytes based on their morphology at a single cell level. The proposed platform provides a novel approach to high throughput and drug screening for different types of heart failure.

Tags

Keywords: Cell ShapeTranscriptomicsSingle CellCardiomyocyteMicropatterned ChipFibronectinCell SortingCell PickerCell CultureCell MorphologyGene ExpressionHigh Throughput

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