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Preparation of Functional Silica Using a Bioinspired Method
JoVE Journal
Chemistry
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JoVE Journal Chemistry
Preparation of Functional Silica Using a Bioinspired Method

Preparation of Functional Silica Using a Bioinspired Method

DOI:
10.3791/57730-v

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08:04 min

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August 01, 2018

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, ,
1Department of Chemical and Biological Engineering,University of Sheffield

Chapters

  • 00:04Title
  • 00:54Preparation of Precursor Solutions (and Optional Encapsulant Solutions)
  • 01:50Silica Synthesis
  • 02:48Acid Elution of the Materials and Silica Separation and Drying
  • 03:53Silicomolybdic Acid Assay on Monomeric Silica Species
  • 04:37Bradford Assay Procedure for Determination of Protein Concentration in Silica
  • 05:28Results: Analysis of Functional Silica Prepared via a Bioinspired Method
  • 06:56Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present a protocol to synthesize bioinspired silica materials and immobilize enzymes therein. Silica is synthesized by combining sodium silicate and an amine 'additive', which neutralize at a controlled rate. Material properties and function can be altered either by in situ enzyme immobilization or post-synthetic acid elution of encapsulated additives.

Tags

Keywords: Bioinspired SilicaFunctional SilicaRapid SynthesisEnzyme EncapsulationPH-dependent Silica FormationSodium SilicatePentaethylene HexamineHydrochloric Acid

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