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Journal / Developmental Biology / En forenklet og effektiv metode å isolere primære …
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Developmental Biology
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JoVE Journal Developmental Biology
En forenklet og effektiv metode å isolere primære menneskelige keratinocytter fra voksen hud vev
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En forenklet og effektiv metode å isolere primære menneskelige keratinocytter fra voksen hud vev

Article DOI: 10.3791/57784-v • 06:17 min • August 25th, 2018
August 25th, 2018 •
1, 1,2, 1, 1, 3, 1, 1,2
1Shandong Provincial Key Laboratory of Oral Tissue Regeneration and Laboratory for Tissue Engineering and Regeneration, School of Stomatology, Shandong University, 2Suzhou Institute of Shandong University, 3Department of Urology, Qilu Hospital of Shandong University

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Her presenterer vi en protokoll for å effektivt isolere primære menneskelige keratinocytter fra voksen hud vev. Denne metoden forenkler konvensjonelle prosedyren ved hjelp av ROCK hemmer Y-27632 i inoculation medium spontant skille epidermal celler fra dermal celler.

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Utviklingspsykologi biologi problemet 138 menneskelige keratinocytter epidermal stamceller dermal celler primære hud cellen aisolering keratinocyte kultur Y-27632 Rho-assosiert kinase inhibitor hud regenerasjon
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