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JoVE Journal Developmental Biology
Isolasjon, kultur og adipogen induksjon av Neural Crest Original Adipose-Avledet stamceller fra periaortisk fettvev
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Isolasjon, kultur og adipogen induksjon av Neural Crest Original Adipose-Avledet stamceller fra periaortisk fettvev

Article DOI: 10.3791/60691-v • 08:31 min • March 2nd, 2020
March 2nd, 2020 •
*1, *2, 3, 3, 4, 5, 4, 5, 1
1Department of Cardiology, Shanghai Chest Hospital, Shanghai Jiao Tong University, 2Shanghai Institute of Nutrition and Health, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, 3Shanghai Key Laboratory of Hypertension, Shanghai Institute of Hypertension, Ruijin Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, 4Department of Cardiology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, 5Department of Cardiology, Shanghai Key Laboratory of Clinical Geriatric Medicine, Huadong Hospital Affiliated to Fudan University
* These authors contributed equally

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Vi presenterer en protokoll for isolasjon, kultur og adipogen induksjon av neural crest avledet fettavledede stamceller (NCADSCs) fra periaortisk fettvev av Wnt-1 Cre+/-; Rosa26RFP/+ mus. NCADSCs kan være en lett tilgjengelig kilde til ADSCs for modellering av adipogenese eller lipogenesis in vitro.

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Utviklingsbiologi Utgave 157 neural crest celle Wnt-1 mus periaortisk fettvev fettavledede stromalceller stromal vaskulær fraksjon cellekultur adipogen induksjon
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