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In Vitro Rearing of Solitary Bees: A Tool for Assessing Larval Risk Factors
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JoVE Journal Environment
In Vitro Rearing of Solitary Bees: A Tool for Assessing Larval Risk Factors

In Vitro Rearing of Solitary Bees: A Tool for Assessing Larval Risk Factors

DOI:
10.3791/57876-v

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08:50 min

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July 16, 2018

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, , ,
1Department of Entomology,University of Wisconsin-Madison, 2Department of Bacteriology,University of Wisconsin-Madison, 3Department of Horticulture,University of Wisconsin-Madison, 4Instituto Nacional de Investigaciones Forestales Agricolas y Pecuarias, 5USDA-ARS, Vegetable Crop Research Unit

Chapters

  • 00:04Title
  • 00:57Harvest Eggs and Host Pollen Provisions from Osmia Reeds
  • 02:10Prepare Pollen Provisors and Treatment Plates
  • 04:24Rearing and Observing Larvae on Treatment Plates
  • 06:08Results: Fungicide and Non-host Pollen Treatments on Developmental Time, Prepupal Biomass
  • 07:23Conclusion

Summary

Automatic Translation

Automatic Translation

Fungicide sprays on flowering plants may expose solitary bees to high concentrations of pollen-borne fungicide residues. Using laboratory-based experiments involving in vitro-reared bee larvae, this study investigates the interactive effects of consuming fungicide-treated pollen derived from host and non-host plants.

Tags

Keywords: In Vitro RearingSolitary BeesLarval Risk FactorsMicrobial SymbiontsOsmiaPollen ProvisionsNon-host PollenSterilized InstrumentsWeigh BoatCulture Plate

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