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05:52 min
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June 28, 2018
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Summary
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Självlysande bakterier reglerar ljus produktion genom olika mekanismer, såsom quorum sensing. Denna nya metod kan i situ utredningen av Mareld och sambandet mellan den ljusa utsläppet till cell densiteten. En konstgjord självlysande Escherichia coli systemet tillåter karakterisering av lux operons, Lux proteiner och deras samspel.
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Cite this Article
Brodl, E., Niederhauser, J., Macheroux, P. In Situ Measurement and Correlation of Cell Density and Light Emission of Bioluminescent Bacteria. J. Vis. Exp. (136), e57881, doi:10.3791/57881 (2018).
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