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Journal / Bioengineering / Korrelationsmaalinger lys Elektron Mikroskopi…
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Bioengineering

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JoVE Journal Bioengineering
Korrelationsmaalinger lys Elektron Mikroskopi (CLEM) til sporing og Imaging Viral Protein associeret strukturer i Cryo-immobiliserede celler
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Korrelationsmaalinger lys Elektron Mikroskopi (CLEM) til sporing og Imaging Viral Protein associeret strukturer i Cryo-immobiliserede celler

Article DOI: 10.3791/58154-v • 09:18 min • September 7th, 2018
September 7th, 2018 •
1, 2, 1, 3, 1, 1, 2,4, 2
1European Molecular Biology Laboratory, 2Department of Infectious Diseases, Molecular Virology, Heidelberg University, 3Central Facility for Electron Microscopy, Ulm University, 4Heidelberg Partner Site, German Center for Infection Research

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En korrelationsmaalinger lys elektronmikroskopi (CLEM) metode kan anvendes på billedet virus-induceret intracellulære strukturer via elektronmikroskopi (EM) i celler, der er tidligere vælges ved lysmikroskopi (LM). LM og EM er kombineret som en hybrid imaging tilgang til at opnå en integreret visning af virus-værtssammenspil.

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Bioteknologi sag 139 højtryk frysning fryse substitution vitrifikation målrettede trimning transmissions elektronmikroskopi safir diske cellekultur virus infektion virus-værtssammenspil ultrastruktur virus-induceret cellulære ændringer.
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