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JoVE Journal Bioengineering
Fra celler til cellefri proteinsyntese inden for 24 timer ved hjælp af cellefri autoinduktionsarbejdsgang
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Fra celler til cellefri proteinsyntese inden for 24 timer ved hjælp af cellefri autoinduktionsarbejdsgang

Article DOI: 10.3791/62866-v • 06:08 min • July 22nd, 2021
July 22nd, 2021 •
1,2, 1,2, 1,2, 1,2
1Department of Chemistry and Biochemistry, California Polytechnic State University San Luis Obispo, 2Center for Application in Biotechnology, California Polytechnic State University San Luis Obispo

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Dette arbejde beskriver fremstillingen af celleekstrakt fra Escherichia coli (E. coli) efterfulgt af cellefri proteinsyntese (CFPS) reaktioner på under 24 timer. Forklaring af den cellefri autoinduktion (CFAI) protokol beskriver forbedringer foretaget for at reducere forskertilsyn og øge mængderne af opnået celleekstrakt.

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