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Journal / Immunology and Infection / Generering af rekombinant aviær Herpesvirus…
JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
Generering af rekombinant aviær Herpesvirus vektorer med CRISPR/Cas9 gen redigering
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Generering af rekombinant aviær Herpesvirus vektorer med CRISPR/Cas9 gen redigering

Article DOI: 10.3791/58193-v • 12:21 min • January 7th, 2019
January 7th, 2019 •
1,2, 1, 1, 1, 1, 1, 2, 1, 1
1The Pirbright Institute & UK-China Centre of Excellence for Research on Avian Diseases, 2Binzhou Animal Science and Veterinary Medicine Academy & UK-China Centre of Excellence for Research on Avian Diseases

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Herpesvirus af kalkuner (HVT) er udbredt som en vektor platform for generation af rekombinante vacciner mod en række fjerkræsygdomme. Denne artikel beskriver en enkel og hurtig tilgang for generation af rekombinant HVT-vektoriserede vacciner ved hjælp af en integreret NHEJ-CRISPR/Cas9, og Cre-Lox system.

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Immunologi og infektion spørgsmålet 143 CRISPR/Cas9 NHEJ Cre-LoxP HVT rekombinant vaccine fjerkræsygdomme
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