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Neurogenesis bruke P19 embryo kreftceller
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Neurogenesis Using P19 Embryonal Carcinoma Cells
DOI:

07:46 min

April 27, 2019

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Chapters

  • 00:04Title
  • 00:27Culture Maintenance and Sub-culturing Cells
  • 02:13Trypsin Digestion
  • 03:29Aggregate Generation
  • 04:34Aggregate Dissociation
  • 05:47Plating Cells
  • 06:31Results: Neuronal Differentiation of P19 Cell Line
  • 07:23Conclusion

Summary

Automatic Translation

Den P19 musen embryonale kreftcelle linje (P19 cellelinje) er mye brukt for å studere den molekylære mekanismen av neurogenesis med stor forenkling i forhold til in vivo analyse. Her presenterer vi en protokoll for retinoic acid-indusert neurogenesis i P19 cellelinjen.

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