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使用P19胚胎癌细胞的神经发生
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Neurogenesis Using P19 Embryonal Carcinoma Cells
DOI:

07:46 min

April 27, 2019

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Chapters

  • 00:04Title
  • 00:27Culture Maintenance and Sub-culturing Cells
  • 02:13Trypsin Digestion
  • 03:29Aggregate Generation
  • 04:34Aggregate Dissociation
  • 05:47Plating Cells
  • 06:31Results: Neuronal Differentiation of P19 Cell Line
  • 07:23Conclusion

Summary

Automatic Translation

P19小鼠胚胎癌细胞系(P19细胞系)与体内分析相比,广泛用于神经发生分子机制的研究,具有极大的简化性。在这里,我们提出了P19细胞系中视黄酸引起的神经发生方案。

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