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1Department of Experimental Embryology, Institute of Genetics and Animal Breeding,Polish Academy of Sciences, 2Department of Regenerative Medicine,Maria Skłodowska-Curie Institute – Oncology Center, 3Department of Genomics and Biodiversities, Institute of Genetics and Animal Breeding,Polish Academy of Sciences
Chapters
- 00:04Title
- 00:27Culture Maintenance and Sub-culturing Cells
- 02:13Trypsin Digestion
- 03:29Aggregate Generation
- 04:34Aggregate Dissociation
- 05:47Plating Cells
- 06:31Results: Neuronal Differentiation of P19 Cell Line
- 07:23Conclusion
Den P19 mus embryonale karcinom cellelinje (P19 cellelinje) er almindeligt anvendt til at studere den molekylære mekanisme af neurogenese med stor forenkling i forhold til in vivo-analyse. Her præsenterer vi en protokol for retinoinsyre-induceret neurogenese i P19 cellelinje.
