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1Department of Experimental Embryology, Institute of Genetics and Animal Breeding,Polish Academy of Sciences, 2Department of Regenerative Medicine,Maria Skłodowska-Curie Institute – Oncology Center, 3Department of Genomics and Biodiversities, Institute of Genetics and Animal Breeding,Polish Academy of Sciences
Chapters
- 00:04Title
- 00:27Culture Maintenance and Sub-culturing Cells
- 02:13Trypsin Digestion
- 03:29Aggregate Generation
- 04:34Aggregate Dissociation
- 05:47Plating Cells
- 06:31Results: Neuronal Differentiation of P19 Cell Line
- 07:23Conclusion
La línea celular de carcinoma embrionario de ratón P19 (línea celular P19) es ampliamente utilizada para estudiar el mecanismo molecular de la neurogénesis con gran simplificación en comparación con el análisis in vivo. Aquí, presentamos un protocolo para la neurogénesis inducida por ácido retinoico en la línea celular P19.
