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Journal / Immunology and Infection / Generation von Knock-out-Grund- und erweiterten…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Generation von Knock-out-Grund- und erweiterten menschlichen NK-Zellen mit Cas9 Ribonucleoproteins
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Generation von Knock-out-Grund- und erweiterten menschlichen NK-Zellen mit Cas9 Ribonucleoproteins

Article DOI: 10.3791/58237-v • 07:26 min • June 14th, 2018
June 14th, 2018 •
1, 2, 1, 3, 1, 1, 1, 1, 1, 1, 1, 3, 1
1Center for Childhood Cancer and Blood Disease, Nationwide Children's Hospital, 2Nuffield Division of Clinical Laboratory Sciences, Radcliffe Department of Medicine, University of Oxford, 3Center for Clinical and Translational Research, Nationwide Children's Hospital Research Institute

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Hier präsentieren wir Ihnen ein Protokoll zur primären oder erweiterten menschlichen natürlichen (NK) Killerzellen mit Cas9 Ribonucleoproteins (Cas9/RNPs) genetisch zu verändern. Mithilfe dieses Protokolls erzielten wir menschliche NK-Zellen für Umwandlung Wachstumsfaktor-b-Rezeptor 2 (TGFBR2) und Hypoxanthin-Phosphoribosyltransferase 1 (HPRT1) mangelhaft.

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Immunologie und Infektion Ausgabe 136 menschliche primäre NK-Zellen Human erweitert NK-Zellen CRISPR/Cas9 Cas9/RNPs gRNAs TGFBR2 beständig TGFβ NK-Zellen
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