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piggyBac Transposon-System Modifikation von primären menschlichen T-Zellen
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1Program in Translational Biology and Molecular Medicine,Baylor College of Medicine , 2Department of Medicine, Division of Nephrology,Baylor College of Medicine , 3Department of Immunology and Pathology,Shinshu University School of Medicine, 4Center for Cell and Gene Therapy,Baylor College of Medicine , 5Department of Pediatrics,Baylor College of Medicine , 6Program in Cell and Molecular Biology,Baylor College of Medicine , 7Department of Molecular Virology and Microbiology,Baylor College of Medicine , 8Michael E. DeBakey VA Medical Center
Chapters
- 00:05Title
- 01:39Day 0: Isolation of PBMCs from Human Blood
- 03:23Day 1: Coating Plates with anti-CD28 and anti-CD3 Antibody for Stimulating T Cells
- 03:59Day 1: Nucleofection of Unstimulated T Cells
- 05:54Day 2: Nonspecific Stimulation of T Cells
- 07:28Day 7: Analysis of Gene Expression
- 08:05Results: piggyBac-mediated Gene Modification of Human T Cells
- 09:19Conclusion
Wir beschreiben ein Verfahren zur genetischen Veränderung primären humanen T-Zellen mit einem Transgen unter Verwendung des nicht-viralen<em> PiggyBac</em> Transposon-System. T-Zellen zur Verwendung der modifizierte<em> PiggyBac</em> Transposonsystem Ausstellung stabile Transgenexpression.
