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Lentiviral vektor-medieret genterapi af hepatocytter Ex Vivo til autolog Transplantation hos svin
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JoVE Journal Medicine
Lentiviral Vector-mediated Gene Therapy of Hepatocytes Ex Vivo for Autologous Transplantation in Swine

Lentiviral vektor-medieret genterapi af hepatocytter Ex Vivo til autolog Transplantation hos svin

DOI:
10.3791/58399-v

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09:54 min

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November 04, 2018

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, , , , , , , , , ,
1Department of Surgery,Mayo Clinic, 2Midwest Fetal Care Center,Children’s Hospitals and Clinics of Minnesota, 3Department of Surgery,Johns Hopkins, 4Pediatric Surgical Associates

Chapters

  • 00:04Title
  • 01:22Laparoscopic Partial Hepatectomy
  • 03:28Hepatocyte Isolation and Transplantation
  • 07:24Results: Representative Lentiviral Vector-mediated Hepatocyte Liver Function Rescue
  • 09:09Conclusion

Summary

Automatic Translation

Automatic Translation

Denne protokol er beregnet til at beskrive svin hepatocyt isolation og ex vivo gen levering for at helbrede modeller af metaboliske sygdomme via autologe celler transplantation. Selv om netop denne model har unikke fordele, der favoriserer succesfulde terapi, er ansøgningen en relevant foundation til at løse flere sygdomme og indikationer.

Tags

Lentiviral VectorGene TherapyHepatocytesEx VivoAutologous TransplantationInborn Errors Of MetabolismLiver DiseaseHereditary Tyrosinemia Type 1Surgical ProcedureLaparoscopic TechniqueLiver Resection

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