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Separación de espinacas tilacoides complejos de proteínas por electroforesis en Gel verde nativo y caracterización de banda utilizando conteo de fotones solo Time-Correlated
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Chapters
- 00:04Title
- 00:50Isolation of Crude Thylakoid Membranes from Spinach Leaves
- 02:51Solubilization of Thylakoid Membranes for Loading onto Native Gels and Separation of Solubilized Thylakoid Proteins by Native Gel Electrophoresis
- 04:02Excision of Thylakoid Complex Bands from Native Green Gels and Collection of Room Temperature Steady-State Fluorescence Spectra
- 05:01Time-correlated Single Photon Counting of Green Gel Bands and Data Analysis
- 06:07Results: Native Green Gel Electrophoresis and Band Characterization
- 08:14Conclusion
Aquí presentamos un protocolo para separar complejos de tilacoides solubilizado por electroforesis nativa en verde. Bandas de gel verde posteriormente se caracterizan por tiempo correlacionó solo fotón contando (TCSPC) y pasos básicos para el análisis de los datos se proporcionan.
