Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
JoVE Journal
Environment
Author Produced

This content is Open Access.

Detection of Tilapia Lake Virus Using Conventional RT-PCR and SYBR Green RT-qPCR
 

Detection of Tilapia Lake Virus Using Conventional RT-PCR and SYBR Green RT-qPCR

Article doi: 10.3791/58596
November 10th, 2018

Summary November 10th, 2018

This protocol diagnoses Tilapia Lake Virus (TiLV) in tilapia tissues using RT-PCR methodologies. The entire method is described from tissue dissection to total RNA extraction, followed by cDNA synthesis and detection of TiLV by either conventional PCR or quantitative PCR using dsDNA binding a fluorescent binding dye.

Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
simple hit counter