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Transdermal Measurement of Glomerular Filtration Rate in Mice
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JoVE Journal Medicine
Transdermal Measurement of Glomerular Filtration Rate in Mice

Transdermal Measurement of Glomerular Filtration Rate in Mice

DOI:
10.3791/58520-v

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07:25 min

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October 21, 2018

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, , , , , , ,
1Division of Nephrology, Department of Medicine,Vanderbilt University Medical Center, 2Department of Cellular and Molecular Physiology,University of Liverpool, 3MediBeacon GmbH, 4Department of Veterinary Pathology and Public Health, Institute of Veterinary Science,University of Liverpool

Chapters

  • 00:04Title
  • 01:14Mouse and Transdermal Glomerular Filtration Rate (tGFR) Monitor Preparation
  • 02:18Transdermal GFR Monitor Attachment
  • 03:26FITC-sinistrin Injection and tGFR Measurement
  • 05:11Results: Representative GFR Monitoring
  • 06:50Conclusion

Summary

Automatic Translation

Automatic Translation

Here we describe a protocol to measure glomerular filtration rate (GFR) in conscious, freely moving mice using a transdermal GFR monitor.

Tags

Keywords: Transdermal MeasurementGlomerular Filtration RateTGFRConscious MiceRenal FunctionGFR DeviceAdhesive Patch70% EthanolData Acquisition

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