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Journal / Biochemistry / Strukturelle undersøgelser af makromolekyler i…
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Biochemistry

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JoVE Journal Biochemistry
Strukturelle undersøgelser af makromolekyler i løsning ved hjælp af små vinkel X-Ray spredning
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Strukturelle undersøgelser af makromolekyler i løsning ved hjælp af små vinkel X-Ray spredning

Article DOI: 10.3791/58538-v • 07:19 min • November 5th, 2018
November 5th, 2018 •
1, 2, 2,4, 1,3,4
1Alberta RNA Research and Training Institute, Department of Chemistry and Biochemistry, University of Lethbridge, 2Department of Biochemistry, University of Alberta, 3Department of Microbiology, Immunology and Infectious Diseases, Cumming School of Medicine, University of Calgary, 4DiscoveryLab, Faculty of Medicine & Dentistry, University of Alberta

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Vi præsenterer her, hvordan lille vinkel X-Ray spredning (SAXSA) kan udnyttes til at få oplysninger om lav opløsning konvolutter repræsenterer de makromolekylære strukturer. Når det bruges sammen med høj opløsning strukturelle teknikker såsom røntgenkrystallografi og Kernemagnetisk resonans, kan SAXSA levere nærmere indsigt i flere domæner proteiner og makromolekylære komplekser i-løsning.

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Biokemi sag 141 ekstracellulære matrix protein hybrid løsning struktur laminin flere domæner protein nidogen protein interaktion protein struktur strukturel domæne lille vinkel X-ray spredning tre-dimensionelle model
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