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A Method for Measuring Metabolism in Sorted Subpopulations of Complex Cell Communities Using Stable Isotope Tracing
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Biochemistry
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JoVE Journal Biochemistry
A Method for Measuring Metabolism in Sorted Subpopulations of Complex Cell Communities Using Stable Isotope Tracing

A Method for Measuring Metabolism in Sorted Subpopulations of Complex Cell Communities Using Stable Isotope Tracing

DOI:
10.3791/55011-v

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07:41 min

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February 04, 2017

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, , , , ,
1Department of Medicine, Unit of Computational Medicine,Karolinska Institutet, 2Center for Molecular Medicine,Karolinska Institutet, 3Department of Medical Biochemistry and Biophysics, Division of Physiological Chemistry 2,Karolinska Institutet, 4Department of Medicine,University of California San Diego

Chapters

  • 00:05Title
  • 00:52Metabolite Extraction from a Dish
  • 01:53Metabolite Extraction of Sorted Cell
  • 04:26Data Analysis
  • 05:22Results: Representative Mas Isotopomer Distribution Validation and Cell Cycle Phase Difference
  • 06:50Conclusion

Summary

Automatic Translation

Automatic Translation

This article describes a method for studying cellular metabolism in complex communities of multiple cell types, using a combination of stable isotope tracing, cell sorting to isolate specific cell types, and mass spectrometry.

Tags

Metabolite ExtractionCell SortingStable Isotope TracingMetabolismCell SubpopulationsCell CultureMass Spectrometry

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