JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Neuroscience
/
一种微琼脂盐桥电极分析重组膜蛋白质子周转率
JoVE Journal
Neuroscience
This content is Free Access.
JoVE Journal
Neuroscience
A Micro-agar Salt Bridge Electrode for Analyzing the Proton Turnover Rate of Recombinant Membrane Proteins
Please note that all translations are automatically generated.
Click here for the English version.
一种微琼脂盐桥电极分析重组膜蛋白质子周转率
DOI:
10.3791/58552-v
•
08:09 min
•
January 07, 2019
•
Jürgen Kreiter
,
Elena E. Pohl
1
Department of Physiology and Biophysics
,
University of Veterinary Medicine
Chapters
00:04
Title
00:44
Preparation of the Micro-agar Salt Bridge Electrode
05:26
Measurement of the Electrical Parameters of the Membrane Reconstituted with Recombinant Protein
06:23
Results: Comparison of Membrane Measurements Made with AgCl and Salt Bridge Electrodes
07:41
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
在电生理测量中, 扩散电位的存在通过改变电极电位来干扰反向电位的精确测量。使用微琼脂盐桥, 扩散电位的影响最小化, 从而可以更精确地测量重组重组膜蛋白的基板周转数。
Tags
Micro-agar Salt Bridge Electrode
Membrane Proteins
Transporters
Channels
Substrate Turnover Rate
Protein Activity
Physiological Conditions
Pathological Conditions
Micro-capillary Pipette Tips
Silver Wire
Potassium Chloride Solution
Ethanol
DC Power Supply
Agarose
Salt Solution
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
脊髓电二:外吸电极的制作
使用调频染料培养的小脑颗粒神经突触小泡池补充的定量分析
横向扩散和培养的神经元膜蛋白的胞吐评估使用荧光恢复和荧光损失漂白
设计和超轻重量的制作,可调节的多电极电生理记录小鼠探头
突触质膜和突触后密度蛋白采用不连续蔗糖梯度准备
使用生物素化测定原代星形细胞培养物中蛋白质的细胞表面表达和吞噬率
多柔性聚合物电极阵列的慢性植入
使用 PRIMO 微模式系统对蛋白质的光诱导分子吸附,研究细胞对细胞外基质蛋白的反应
Measuring Membrane Lipid Turnover with the pH-sensitive Fluorescent Lipid Analog ND6
使用双极电极通过电点燃杏仁核创建颞叶癫痫小鼠模型
Read Article