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一种微琼脂盐桥电极分析重组膜蛋白质子周转率
JoVE Journal
Neuroscience
This content is Free Access.
JoVE Journal Neuroscience
A Micro-agar Salt Bridge Electrode for Analyzing the Proton Turnover Rate of Recombinant Membrane Proteins
DOI:

08:09 min

January 07, 2019

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Chapters

  • 00:04Title
  • 00:44Preparation of the Micro-agar Salt Bridge Electrode
  • 05:26Measurement of the Electrical Parameters of the Membrane Reconstituted with Recombinant Protein
  • 06:23Results: Comparison of Membrane Measurements Made with AgCl and Salt Bridge Electrodes
  • 07:41Conclusion

Summary

Automatic Translation

在电生理测量中, 扩散电位的存在通过改变电极电位来干扰反向电位的精确测量。使用微琼脂盐桥, 扩散电位的影响最小化, 从而可以更精确地测量重组重组膜蛋白的基板周转数。

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