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一种微琼脂盐桥电极分析重组膜蛋白质子周转率
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
A Micro-agar Salt Bridge Electrode for Analyzing the Proton Turnover Rate of Recombinant Membrane Proteins
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一种微琼脂盐桥电极分析重组膜蛋白质子周转率

DOI:
10.3791/58552-v

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08:09 min

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January 07, 2019

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1Department of Physiology and Biophysics,University of Veterinary Medicine

Chapters

  • 00:04Title
  • 00:44Preparation of the Micro-agar Salt Bridge Electrode
  • 05:26Measurement of the Electrical Parameters of the Membrane Reconstituted with Recombinant Protein
  • 06:23Results: Comparison of Membrane Measurements Made with AgCl and Salt Bridge Electrodes
  • 07:41Conclusion

Summary

Automatic Translation

Automatic Translation

在电生理测量中, 扩散电位的存在通过改变电极电位来干扰反向电位的精确测量。使用微琼脂盐桥, 扩散电位的影响最小化, 从而可以更精确地测量重组重组膜蛋白的基板周转数。

Tags

Micro-agar Salt Bridge ElectrodeMembrane ProteinsTransportersChannelsSubstrate Turnover RateProtein ActivityPhysiological ConditionsPathological ConditionsMicro-capillary Pipette TipsSilver WirePotassium Chloride SolutionEthanolDC Power SupplyAgaroseSalt Solution

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