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JoVE Journal Biology

LDL 콜레스테롤 이해 분석 결과 라이브 셀 이미징 분석 셀을 사용 하 여 상태 모니터링

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LDL 콜레스테롤 이해 분석 결과 라이브 셀 이미징 분석 셀을 사용 하 여 상태 모니터링

Article DOI: 10.3791/58564-v • 08:45 min • November 17th, 2018

November 17th, 2018 •

Portia Ritter*^1,2, Keyvan Yousefi*^1,3, Juliana Ramirez⁴, Derek M. Dykxhoorn^4,5, Armando J. Mendez⁶, Lina A. Shehadeh^1,2,7,8

¹Interdisciplinary Stem Cell Institute, University of Miami Leonard M. Miller School of Medicine, ²Department of Medicine, Division of Cardiology, University of Miami Leonard M. Miller School of Medicine, ³Department of Molecular and Cellular Pharmacology, University of Miami Leonard M. Miller School of Medicine, ⁴Dr. John T. Macdonald Foundation Department of Human Genetics, University of Miami Leonard M. Miller School of Medicine, ⁵John P. Hussman Institute for Human Genomics, University of Miami Leonard M. Miller School of Medicine, ⁶Department of Medicine, Division of Endocrinology, Metabolism and Endocrinology and the Diabetes Research Institute, University of Miami Leonard M. Miller School of Medicine, ⁷Vascular Biology Institute, University of Miami Leonard M. Miller School of Medicine, ⁸Peggy and Harold Katz Family Drug Discovery Center, University of Miami Leonard M. Miller School of Medicine

* These authors contributed equally

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이 프로토콜에는 라이브 셀 이미징 다양 한 셀 형식에 시스템을 사용 하 여 실시간으로 유입 속도와 LDL 콜레스테롤 흡수를 측정 하는 효율적인 접근 방식을 제공 합니다. 이 기술은 세포 형태학 및 그러므로 잠재적인 세포 독성에 대 한 모니터링 하면서 LDL 유입에 영향을 미치는 화합물의 약리 활동 화면 플랫폼을 제공 합니다.

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생물학 문제점 141 저밀도 LDLR LDL 유입 LDL의 통풍 관 Dynasore Simvastatin 재조합 PCSK9 dynamin 지질 콜레스테롤 동맥 경화.

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