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Etablering og analyse av Tumor Slice Explants er en forutsetning for diagnostiske tester
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal Immunology and Infection
Establishment and Analysis of Tumor Slice Explants As a Prerequisite for Diagnostic Testing
DOI:

11:00 min

November 29, 2018

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Chapters

  • 00:04Title
  • 00:42Collection of Tumor-bearing Lungs
  • 02:08Generation and Cultivation of Precision-cut Lung Tumor Slices, and Treatment with Inhibitors
  • 05:58Fixation and Processing of the Tissue Slices
  • 07:27Processing and Analysis of Formalin-fixed and Paraffin-embedded (FFPE) Tissues
  • 08:33Results: Assessment of Viability, Specific Marker Expression, and Targeted Drug Treatment on Adenocarcinoma Tissue Slices
  • 10:26Conclusion

Summary

Automatic Translation

Vi tilbyr en metode for generasjon, dyrking og systematisk analyse av organotypic skiver avledet fra murine lunge svulst. Vi beskriver også hvordan å optimalisere for skive tykkelse, og velge narkotika konsentrasjoner å behandle svulst skiver.

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