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Immunology and Infection
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Oprettelse og analyse af Tumor skive Explants som en forudsætning for diagnostiske test
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal Immunology and Infection
Establishment and Analysis of Tumor Slice Explants As a Prerequisite for Diagnostic Testing

Oprettelse og analyse af Tumor skive Explants som en forudsætning for diagnostiske test

DOI:
10.3791/58569-v

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11:00 min

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November 29, 2018

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, , , , ,
1Institute for Molecular Medicine Finland (FIMM), HiLIFE,University of Helsinki

Chapters

  • 00:04Title
  • 00:42Collection of Tumor-bearing Lungs
  • 02:08Generation and Cultivation of Precision-cut Lung Tumor Slices, and Treatment with Inhibitors
  • 05:58Fixation and Processing of the Tissue Slices
  • 07:27Processing and Analysis of Formalin-fixed and Paraffin-embedded (FFPE) Tissues
  • 08:33Results: Assessment of Viability, Specific Marker Expression, and Targeted Drug Treatment on Adenocarcinoma Tissue Slices
  • 10:26Conclusion

Summary

Automatic Translation

Automatic Translation

Vi leverer en metode for generation, dyrkning og systematisk analyse af organotypic skiver afledt af murine lunge tumorer. Vi beskriver også, hvordan du optimerer til skive tykkelse, og hvordan du vælger stof koncentrationer til behandling af tumor skiver.

Tags

Tumor Slice ExplantsPreclinical ResearchDrug Response StudiesMurine Lung TumorsVibratomeHank’s Balanced Salt SolutionEuthanized MouseLungHeartCyanoacrylate Adhesive

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