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Journal / Environment / Reproducerbara dsRNA Mikroskop och ovipositionen…
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JoVE Journal Environment
Reproducerbara dsRNA Mikroskop och ovipositionen bioassayen i myggor och hus flugor
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Reproducerbara dsRNA Mikroskop och ovipositionen bioassayen i myggor och hus flugor

Article DOI: 10.3791/58650-v • 08:41 min • November 8th, 2018
November 8th, 2018 •
1, 2,3, 1, 1,2, 1
1USDA/ARS Center for Medical, Agricultural, and Veterinary Entomology, 2CMAVE Detachment, Navy Entomology Center of Excellence, 3Lovelace Respiratory Research Institute

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Det här protokollet beskriver en Mikroskop metod som vi har standardiserat och använt i flera år för att leverera vissa mängder nukleinsyror direkt till hemolymph myggor och hus flugor. Detta protokoll resulterar i minimal injektion dödlighet och tillåter dos korrelerade mätningar av fruktsamhet.

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