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Journal / Genetics / Adskille bakterier af kapsel beløb ved hjælp af…
JoVE Journal
Genetics

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JoVE Journal Genetics
Adskille bakterier af kapsel beløb ved hjælp af en diskontinuerlig tæthed farveforløb
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Adskille bakterier af kapsel beløb ved hjælp af en diskontinuerlig tæthed farveforløb

Article DOI: 10.3791/58679-v • 05:52 min • January 7th, 2019
January 7th, 2019 •
1, 1, 1, 1, 1,2
1Wellcome Sanger Institute, Wellcome Genome Campus, 2Department of Medicine, University of Cambridge

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Vi demonstrere brugen af ikke-vedvarende tæthed gradienter at adskille bakteriel befolkning baseret på kapsel produktion. Denne metode bruges til at sammenligne kapsel beløb mellem kulturer, isolere mutanter med en specifik kapsel fænotype eller identificere kapsel regulatorer. Beskrevet her er optimering og drift af analysen.

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Genetik spørgsmålet 143 diskontinuert tæthed farveforløb kapsel TraDIS hypermucoidy Klebsiella
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