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Journal / Genetics / CRISPR-mediert genomet redigering av Human Fungal …
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Genetics

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JoVE Journal Genetics
CRISPR-mediert genomet redigering av Human Fungal patogen Candida albicans
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CRISPR-mediert genomet redigering av Human Fungal patogen Candida albicans

Article DOI: 10.3791/58764-v • 09:56 min • November 14th, 2018
November 14th, 2018 •
*1, *1, 2, 1
1Department of Biology, Ball State University, 2Whitehead Institute for Biomedical Research
* These authors contributed equally

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Effektiv genomet engineering av Candida albicans er avgjørende for å forstå den patogenesen og utvikling av therapeutics. Her beskrev vi en protokoll for å raskt og nøyaktig redigere C. albicans genomet bruker CRISPR. Protokollen lar etterforskerne å introdusere en rekke genetiske modifikasjoner inkludert punkt mutasjoner, innsettinger og slettinger.

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Genetikk problemet 141 CRISPR Candida albicans genomet redigering transformasjon Cas9 patogen sopp
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