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Journal / Genetics / CRISPR-Cas9 genomredigering av rotteembryoer ved…
JoVE Journal
Genetics
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JoVE Journal Genetics
CRISPR-Cas9 genomredigering av rotteembryoer ved bruk av adenoassosiert virus (AAV) og 2-celleembryoelektroporering
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CRISPR-Cas9 genomredigering av rotteembryoer ved bruk av adenoassosiert virus (AAV) og 2-celleembryoelektroporering

Article DOI: 10.3791/66069-v • 08:24 min • March 15th, 2024
March 15th, 2024 •
1,2,4, 2, 3, 3, 3, 1,2,3,4
1Animal Modeling Core, University of Missouri, 2Comparative Medicine Program, University of Missouri, 3Rat Resource and Research Center, University of Missouri, 4Department of Veterinary Pathobiology, University of Missouri

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Denne protokollen presenterer en optimalisert tilnærming for å produsere genetisk modifiserte rottemodeller. Adenoassosiert virus (AAV) brukes til å levere en DNA-reparasjonsmal, og elektroporering brukes til å levere CRISPR-Cas9-reagenser for å fullføre genomredigeringsprosessen i 2-celleembryo.

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Denne måneden i JoVE utgave 205 adenoassosiert virus (AAV) genomredigering CRISPR-Cas elektroporering 2-celleembryoer knock-in rottedyrmodell
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