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A Human 3D Extracellular Matrix-Adipocyte Culture Model for Studying Matrix-Cell Metabolic Crosstalk
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Bioengineering
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JoVE Journal Bioengineering
A Human 3D Extracellular Matrix-Adipocyte Culture Model for Studying Matrix-Cell Metabolic Crosstalk

A Human 3D Extracellular Matrix-Adipocyte Culture Model for Studying Matrix-Cell Metabolic Crosstalk

DOI:
10.3791/60486-v

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09:04 min

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November 07, 2019

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, , , , , , ,
1Department of Surgery,University of Michigan Medical School, 2Department of Pediatrics and Communicable Diseases,University of Michigan Medical School, 3Graduate Program in Immunology,University of Michigan Medical School, 4Graduate Program in Cellular and Molecular Biology,University of Michigan Medical School, 5Undergraduate Research Opportunity Program,University of Michigan, 6Department of Surgery,Ann Arbor Veterans Affairs Healthcare System

Chapters

  • 00:05Title
  • 01:20Adipose Tissue Procurement and Preadipocyte Isolation
  • 02:51Adipose Tissue ECM Preparation
  • 05:08ECM-Adipocyte Preparation
  • 07:04Results: Genotypic and Phenotypic Characterization of In vitro Differentiated Adipocytes
  • 08:36Conclusion

Summary

Automatic Translation

Automatic Translation

We describe a 3D human extracellular matrix-adipocyte in vitro culture system that permits dissection of the roles of the matrix and adipocytes in contributing to adipose tissue metabolic phenotype.

Tags

Keywords: 3D Extracellular MatrixAdipocyte CultureMetabolic CrosstalkAdipogenic DifferentiationType 2 DiabetesPreadipocyte IsolationDecellularizationCollagenase DigestionECM Preparation

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