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Biology
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大鼠骨髓破骨衣原体的分离、纯化及鉴别
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Biology
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Biology
Isolation, Purification, and Differentiation of Osteoclast Precursors from Rat Bone Marrow
Please note that all translations are automatically generated.
Click here for the English version.
大鼠骨髓破骨衣原体的分离、纯化及鉴别
DOI:
10.3791/58895-v
•
11:11 min
•
May 19, 2019
•
Lining Wang
2
,
Suyang Zheng
2
,
Yang Guo
2
,
Yalan Pan
2
,
Jie Sun
2
,
Weimin Xu
2
,
Jinlan Lu
,
Weidong Li
,
Yong Ma
2,4
1
Laboratory of New Techniques of Restoration & Reconstruction, Institute of Traumatology & Orthopedics
,
Nanjing University of Chinese Medicine
,
2
TCM Nursing Intervention Laboratory of Chronic Disease Key Laboratory
,
Nanjing University of Chinese Medicine
,
3
Jiangsu Key Laboratory of Chinese Medicine Processing, Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing
,
Nanjing University of Chinese Medicine
,
4
Department of Traumatology and Orthopedics
,
Affiliated Hospital of Nanjing University of Chinese Medicine
Chapters
00:04
TItle
00:55
Traditional Method of Isolating Bone Marrow-derived Cells
02:40
Improved Method of Isolating Bone Marrow-derived Cells
04:11
Purification by Density Gradient Centrifugation
05:39
Culture and Differentiation
06:15
Bone Resorption Assay Using Toluidine Blue Staining
08:03
Scanning Electron Microscopy
09:03
Results: Analysis of the Osteoclast Precursors Isolated, Purified, and Differentiated from Rat Bone Marrow
10:40
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
破骨细胞是来源于造血干细胞单核巨噬细胞谱系的组织特异性巨噬细胞多核细胞。该方案描述了如何分离骨髓细胞, 以便获得大量的破骨细胞, 同时降低传统方法中发现的事故风险。
Tags
Osteoclast Precursors
Bone Marrow
Isolation
Purification
Differentiation
Cell Culture
Cell Extraction
Cell Counting
Osteoporosis
Bone Metabolism
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