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JoVE Journal Cancer Research
Evaluering af biomarkører i gliom af Immunhistokemi på Paraffin-Embedded 3D gliom Neurosphere kulturer
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Evaluering af biomarkører i gliom af Immunhistokemi på Paraffin-Embedded 3D gliom Neurosphere kulturer

Article DOI: 10.3791/58931-v • 06:32 min • January 9th, 2019
January 9th, 2019 •
1,2, 2, 1,2, 1,3, 1,2, 1,2, 1,2
1Department of Neurosurgery, University of Michigan Medical School, 2Department of Cell & Developmental Biology, University of Michigan, 3INIBIOLP, Histology B-Pathology B, School of Medicine, UNLP

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Neurospheres dyrkes som 3D kulturer udgør et kraftfuldt værktøj til at studere gliom biologi. Her præsenterer vi en protokol for at udføre Immunhistokemi samtidig bevare 3D-struktur af gliom neurospheres gennem integrering af paraffin. Denne metode gør det muligt for karakterisering af gliom neurosphere egenskaber som stemness og neurale differentiering.

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Kræftforskning spørgsmålet 143 genetisk manipuleret gliom neurospheres 3D-kulturer immunfarvning biomarkører paraffin indlejring
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