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Identificazione dei marcatori di superficie cellulare delle cellule staminali primarie e delle cellule progenitrici mediante l'etichettatura metabolica di Sialoglycan
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1Brain and Spinal Cord Innovative Research Center of Tongji Hospital, School of Life Sciences and Technology,Tongji University and Frontier Science Research Center for Stem Cells of Ministry of Education, 2College of Chemistry and Molecular Engineering, Peking-Tsinghua Center for Life Sciences, Beijing National Laboratory for Molecular Sciences, Synthetic and Functional Biomolecules Center, and Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education,Peking University
Chapters
- 00:05Title
- 01:16Preparation of Mouse Endothelial Culture in Permeable Support Inserts
- 01:55Set-up of Neural-endothelial Co-culture and Ac4ManNAz Labeling System
- 03:25Immunofluorescent Staining of Sialoglycoproteins in Expanded Primary NSPCs and Differentiated Neurons
- 05:25Purification of Sialoglycoproteins from Expanded Primary NSPCs and Differentiated Neurons
- 08:45Results: Identifying Cell Surface Markers by Metabolic Labeling of Sialoglycan
- 10:47Conclusion
Presentato qui è un protocollo che combina un sistema di cocoltura neurale-endoteliale in vitro e l'incorporazione metabolica di sialoglycan con gruppi funzionali bioortogonali per espandere le cellule staminali primarie e progenitrici ed etichettare la loro superficie sialoglycoproteins per l'imaging o l'analisi della spettrometria di massa dei marcatori di superficie cellulare.
