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Identification des marqueurs de surface cellulaire des cellules neurales primaires de tige et de progéniture par l'étiquetage métabolique du sialoglycan
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1Brain and Spinal Cord Innovative Research Center of Tongji Hospital, School of Life Sciences and Technology,Tongji University and Frontier Science Research Center for Stem Cells of Ministry of Education, 2College of Chemistry and Molecular Engineering, Peking-Tsinghua Center for Life Sciences, Beijing National Laboratory for Molecular Sciences, Synthetic and Functional Biomolecules Center, and Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education,Peking University
Chapters
- 00:05Title
- 01:16Preparation of Mouse Endothelial Culture in Permeable Support Inserts
- 01:55Set-up of Neural-endothelial Co-culture and Ac4ManNAz Labeling System
- 03:25Immunofluorescent Staining of Sialoglycoproteins in Expanded Primary NSPCs and Differentiated Neurons
- 05:25Purification of Sialoglycoproteins from Expanded Primary NSPCs and Differentiated Neurons
- 08:45Results: Identifying Cell Surface Markers by Metabolic Labeling of Sialoglycan
- 10:47Conclusion
Présenté ici est un protocole qui combine un système de co-culture neural-endothelial in vitro et l'incorporation métabolique du sialoglycan avec des groupes fonctionnels bioorthogonals pour étendre les cellules neurales primaires de tige et d'ancêtre et étiqueter leur surface sialoglycoproteins pour l'imagerie ou l'analyse de la spectrométrie de masse des marqueurs de surface cellulaire.
